| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.247519 |
| Chromosome: | chromosome 12 |
| Location: | 7540179 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g555650 | (1 of 30) PF03407 - Nucleotide-diphospho-sugar transferase (Nucleotid_trans) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCCACGCCAGCGAGCTGATTACCAAAGT |
| Internal bar code: | GTGCGGGGTCGAAGCTGTTCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 666 |
| LEAP-Seq percent confirming: | 99.5971 |
| LEAP-Seq n confirming: | 4944 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAGGGTGTGGTTTACGTC |
| Suggested primer 2: | CCTGGCTTAAGGGAGAGCTT |