Insertion junction: LMJ.RY0402.247560_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.247560
Chromosome:chromosome 9
Location:6836515
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g409801 (1 of 2) PTHR11373:SF4 - DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE SAMHD1 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAAATGGCCCCCAAACCGACCCTCTGGTT
Internal bar code:GGTTGTGGCTCCGCCAGCATGC

Confirmation - LEAP-Seq

LEAP-Seq distance:552
LEAP-Seq percent confirming:97.8261
LEAP-Seq n confirming:45
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:AAATTGGCATGGATGGTGAT
Suggested primer 2:GTCTCACACATTCGGTGGTG