| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.247564 |
| Chromosome: | chromosome 9 |
| Location: | 3327773 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388430 | (1 of 2) IPR000104//IPR001202 - Antifreeze protein, type I // WW domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGTGAAGGTTGCCTTGGATATCGGATGG |
| Internal bar code: | ATGTCGAAAAGATCGCAGAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 469 |
| LEAP-Seq percent confirming: | 99.5381 |
| LEAP-Seq n confirming: | 431 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCTCTGTGTGATGTGGT |
| Suggested primer 2: | GACTTGACCTGTCCCATCGT |