| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.247592 |
| Chromosome: | chromosome 12 |
| Location: | 2993929 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g501050 | ALDH25,ALD6 | Aldehyde/histidinol dehydrogenase; (1 of 1) 1.1.1.1//1.2.1.10 - Alcohol dehydrogenase / Aldehyde reductase // Acetaldehyde dehydrogenase (acetylating) / Aldehyde dehydrogenase (acylating) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCAACGTCATGGACAAGCTGCCGGCCGA |
| Internal bar code: | CCAGTAGTAGATGATTTTAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 622 |
| LEAP-Seq percent confirming: | 99.1265 |
| LEAP-Seq n confirming: | 6582 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACAAGCCACCCCTTACAG |
| Suggested primer 2: | TTGTGTGTATGTTGGGGGTG |