| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.247644 |
| Chromosome: | chromosome 6 |
| Location: | 4314661 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278257 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGAATGGTGGTTGCGGGCGACTGGGTTG |
| Internal bar code: | ACTACACACCTGTTCCCGAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 569 |
| LEAP-Seq percent confirming: | 99.4092 |
| LEAP-Seq n confirming: | 3702 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTGGTTGTGCTCCTCCAC |
| Suggested primer 2: | AGGCCGTGAGGAAGACCT |