Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.247645 |
Chromosome: | chromosome_14 |
Location: | 733045 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre14.g612700 | DRC7,FAP50 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTGGGACGGTTGCATTGCAGTCAGACTAGA |
Internal bar code: | TCGTATGAGGGTCTGGTTGCCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1010 |
LEAP-Seq percent confirming: | 99.6439 |
LEAP-Seq n confirming: | 3638 |
LEAP-Seq n nonconfirming: | 13 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CACAGCGAGCTAGGGTAAGC |
Suggested primer 2: | GAGGGGGAAGGTGAAAAGAG |