Insertion junction: LMJ.RY0402.247651_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.247651
Chromosome:chromosome 3
Location:7260688
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g207400 (1 of 1) PTHR36846:SF1 - PROTEIN VIAA intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCAGAACCCAAAGCAAACCAACCGAGTG
Internal bar code:CGGGGCCTTCTAGGCTAAACCC

Confirmation - LEAP-Seq

LEAP-Seq distance:807
LEAP-Seq percent confirming:99.2943
LEAP-Seq n confirming:8442
LEAP-Seq n nonconfirming:60
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GCCTTAGAAGGCGTCAACTG
Suggested primer 2:TGTGTGGAGGGGGATTACAT