| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.247661 |
| Chromosome: | chromosome 5 |
| Location: | 618094 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232000 | (1 of 1) IPR001623//IPR011989 - DnaJ domain // Armadillo-like helical | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCTGTTGCGCCGTTGAGGGTTGAAGGGA |
| Internal bar code: | CTCTTTTCCTGGGCAACCGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 455 |
| LEAP-Seq percent confirming: | 99.5958 |
| LEAP-Seq n confirming: | 1232 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATACGGTACTGCCCATCG |
| Suggested primer 2: | GTGTTGTGCGTACCGATGAC |