Insertion junction: LMJ.RY0402.247662_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.247662
Chromosome:chromosome 9
Location:1925596
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g395200 (1 of 1) PF04087 - Domain of unknown function (DUF389) (DUF389) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAGGGCCGCATCTTCTTCAGGCGCTTCGA
Internal bar code:CACGGCGGGTAATGGTTTAGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:548
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTACTGCTTCACGCGACT
Suggested primer 2:CGGGTACAGGTGGAAAAGAA