| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.247662 |
| Chromosome: | chromosome 17 |
| Location: | 5339791 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g736400 | SCC3 | Cohesin complex subunit; (1 of 1) K06671 - cohesin complex subunit SA-1/2 (STAG1_2, SCC3, IRR1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAACGTAAGGAAGTGGCAGACGGTGGTCG |
| Internal bar code: | AAGCCAACCGCCGCATGAATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 111 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAAAAGCACACAGCTCAA |
| Suggested primer 2: | GCCTACCTCAAATACGTGGC |