| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.247690 |
| Chromosome: | chromosome 1 |
| Location: | 1502966 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g007737 | FBT1 | Folate/pteridine transporter 1; (1 of 1) IPR004324//IPR011701//IPR020846 - Folate-biopterin transporter // Major facilitator superfamily // Major facilitator superfamily domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTGCCAGCTCCTGTTGAACCATGTTTGC |
| Internal bar code: | GATACGCTCTACATCTGCGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 560 |
| LEAP-Seq percent confirming: | 89.0436 |
| LEAP-Seq n confirming: | 959 |
| LEAP-Seq n nonconfirming: | 118 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAACGTGACCCCACAAAC |
| Suggested primer 2: | ACATCCCCAACTCTGACAGC |