Insertion junction: LMJ.RY0402.247722_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.247722
Chromosome:chromosome 6
Location:7147534
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g297550 (1 of 1) K14649 - transcription initiation factor TFIID subunit 8 (TAF8) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATTGGTACCATTTCTGTGACACAGTAACA
Internal bar code:ACAACGCTGACTAGGGTTTGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:191
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:68
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TGGTGTTGTGTTGGATGCTT
Suggested primer 2:TCTCTCTCGTGGATTGGCTT