| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.247738 |
| Chromosome: | chromosome 4 |
| Location: | 3855148 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g230144 | SPT1 | Component of serine palmitoyltransferase; (1 of 1) PTHR13693//PTHR13693:SF3 - CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE // LD36009P | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTTTCCCGTCACTCACGAAGGCTCCTTG |
| Internal bar code: | GGAGATTGTTGTCTCTAGCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 819 |
| LEAP-Seq percent confirming: | 99.408 |
| LEAP-Seq n confirming: | 3862 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCTTCTAAATCCGTTTGG |
| Suggested primer 2: | ACCCGTTGTCTCACTCCAAC |