| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.247765 |
| Chromosome: | chromosome 5 |
| Location: | 3055611 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238850 | PHC17 | Putative pherophorin-chlamydomonas homolog | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGATACATGGCACGGGCCACAGGGCACGG |
| Internal bar code: | TTGCGGGTGACAGGTAGCAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 588 |
| LEAP-Seq percent confirming: | 98.9209 |
| LEAP-Seq n confirming: | 275 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCATGAAGGAGGGTAACGG |
| Suggested primer 2: | AGAAAGGGCGTTCTAACCGT |