| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.247805 |
| Chromosome: | chromosome 16 |
| Location: | 7022497 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g678213 | (1 of 1) K01074 - palmitoyl-protein thioesterase (PPT) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCAATCGCGCCTTGGTGAGTTCACGTCG |
| Internal bar code: | ATGGCACCATCAACGATCTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 75 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGTTAGGGGGAAGGATGG |
| Suggested primer 2: | AGCCTTGTGTCTCCGTGTCT |