Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.247856 |
Chromosome: | chromosome 1 |
Location: | 4579167 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g031350 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGAACTCTAGTCACACTCAGCAACACC |
Internal bar code: | TGTCGCTATCCATGTGCATCGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 390 |
LEAP-Seq percent confirming: | 98.7382 |
LEAP-Seq n confirming: | 313 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAATCTTGGGGAACAGCAAT |
Suggested primer 2: | CGTGCTTGATGAAACGCTTA |