| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.247969 |
| Chromosome: | chromosome 17 |
| Location: | 1771647 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g709300 | (1 of 1) PF10009 - Uncharacterized protein conserved in bacteria (DUF2252) (DUF2252) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTTTGACGAGTCCTTTGTCGCCAACTTC |
| Internal bar code: | AAAGCAGGGCTCGCGCAGTTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 646 |
| LEAP-Seq percent confirming: | 98.4211 |
| LEAP-Seq n confirming: | 561 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTACTATCGCGACCTGGC |
| Suggested primer 2: | CTTGACCACCTGCACAGAGA |