Insertion junction: LMJ.RY0402.247985_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.247985
Chromosome:chromosome 10
Location:1904741
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g431900 (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTTATGCGTAGATATGTCTCGTTGTGGG
Internal bar code:GCACATCCCGCTTGTTGCGTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:527
LEAP-Seq percent confirming:98.574
LEAP-Seq n confirming:553
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CCCAACAAGAGCAAGGTGAT
Suggested primer 2:CGTAACACCCCTCATCTCGT