| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.248001 |
| Chromosome: | chromosome 4 |
| Location: | 2901567 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g224500 | (1 of 1) 3.6.3.17//3.6.3.44 - Monosaccharide-transporting ATPase // Xenobiotic-transporting ATPase / Steroid-transporting ATPase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTTGACGCTCTCAGCGGCTCCTGATGCA |
| Internal bar code: | GCCGGGACATTACACCGGATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 531 |
| LEAP-Seq percent confirming: | 99.0361 |
| LEAP-Seq n confirming: | 411 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCGTGGAGCCATATCGAC |
| Suggested primer 2: | GTACTCCCCAGCGGTTTGTA |