Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.248017 |
Chromosome: | chromosome 13 |
Location: | 3522069 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre13.g588000 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTGCCGCTAACCGCCGAGGCGCAGCACA |
Internal bar code: | AATAGGTGCACAACGAGGGCTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 431 |
LEAP-Seq percent confirming: | 99.7886 |
LEAP-Seq n confirming: | 472 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGCAAGGAAAATGCAGGTAT |
Suggested primer 2: | TGTGTGAGGGTGTGTGTGTG |