| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.248025 |
| Chromosome: | chromosome 3 |
| Location: | 5555427 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g186450 | LNM1 | Lysine N-methylase; (1 of 5) PTHR13271//PTHR13271:SF28 - UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCCTAGCCGGAAGCGCACCGCCTCCGCC |
| Internal bar code: | CCTTCGAAACACCATTCCGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1039 |
| LEAP-Seq percent confirming: | 99.6525 |
| LEAP-Seq n confirming: | 2581 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCTTGGATGTGTTGCTCC |
| Suggested primer 2: | AAGAGCGCAAGCTTGGATAA |