| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.248026 |
| Chromosome: | chromosome 9 |
| Location: | 1141710 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400150 | (1 of 2) PF13415//PF13418 - Galactose oxidase, central domain (Kelch_3) // Galactose oxidase, central domain (Kelch_4) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGACGGGCGCAAGGGAAAGGCAGCAACG |
| Internal bar code: | ACCTGACAGTGCAGGGTGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 589 |
| LEAP-Seq percent confirming: | 98.6595 |
| LEAP-Seq n confirming: | 368 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGTCTGAAAGGGACAGCA |
| Suggested primer 2: | AACACTTTGCATCCCCTACG |