| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.248070 |
| Chromosome: | chromosome_9 |
| Location: | 283244 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g392867 | FMG1-B | Flagella membrane glycoprotein, major form | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATGCCTGAATCGGCCTCCCGTTTCTGCAGG |
| Internal bar code: | CTGGAGCGACTTCTTGTTTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 586 |
| LEAP-Seq percent confirming: | 98.8519 |
| LEAP-Seq n confirming: | 9299 |
| LEAP-Seq n nonconfirming: | 108 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGATGGTCAGGAGGGTGT |
| Suggested primer 2: | CGTCGTAATCTACCCCTCCA |