Insertion junction: LMJ.RY0402.248076_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.248076
Chromosome:chromosome 17
Location:2701297
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g717950 PHC28,PHC6 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 6 CDS/intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTAAGTCAACTTCAATAGCTTCCGTCGGAC
Internal bar code:ACGCGGCTGTACCTTGGTGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:268
LEAP-Seq percent confirming:99.3902
LEAP-Seq n confirming:163
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CGTTTCGTCTTGGTCCAAAT
Suggested primer 2:CTCCTGGTAACCCCTCAACA