| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.248084 |
| Chromosome: | chromosome 12 |
| Location: | 9686567 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g556228 | NDA7 | Type-II NADH dehydrogenase; (1 of 5) 1.6.5.9 - NADH:ubiquinone reductase (non-electrogenic) / Ubiquinone reductase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTGACGGGGCCGCGGCCCTCGGCTGCAC |
| Internal bar code: | ATGGACTTCCGGGGGGGGTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 467 |
| LEAP-Seq percent confirming: | 99.5816 |
| LEAP-Seq n confirming: | 952 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAGAGCACAAGGTTCGGA |
| Suggested primer 2: | CATGTCCCTGTGTTCACGTC |