| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.248094 |
| Chromosome: | chromosome_4 |
| Location: | 2143252 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g219700 | BBS9 | Bardet-Biedl syndrome-9 associated protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCACACCCAGTGAGTGCCGGGCACACCGCA |
| Internal bar code: | CGTCTGGTCTAATAATTATGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 855 |
| LEAP-Seq percent confirming: | 99.4704 |
| LEAP-Seq n confirming: | 1127 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTATGCGATGCAGAAAAGC |
| Suggested primer 2: | ACTTACCTGGTGTTCCCACG |