| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.248104 |
| Chromosome: | chromosome 5 |
| Location: | 2177203 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234647 | (1 of 1) K06950 - uncharacterized protein (K06950) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTCGTAGAAATGGTTGATGGTTGTGGAT |
| Internal bar code: | GTGGCTCCAGCCGAGAAGTCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 475 |
| LEAP-Seq percent confirming: | 99.8824 |
| LEAP-Seq n confirming: | 849 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGGCTTCGTTTGGTTGAC |
| Suggested primer 2: | ACCGGTGAGGGCTTTTAACT |