| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.248165 |
| Chromosome: | chromosome 3 |
| Location: | 2486158 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g159650 | EFG6 | (1 of 1) PTHR23115//PTHR23115:SF96 - TRANSLATION FACTOR // SUBFAMILY NOT NAMED; Putative organellar translation elongation factor EFG/EF2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCCCGGGACCCCTTGAGCGTGCCCCTTG |
| Internal bar code: | CGGGAACGACGGTCAGGGCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 611 |
| LEAP-Seq percent confirming: | 98.4716 |
| LEAP-Seq n confirming: | 1353 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAATGGATTGCAACCTGAA |
| Suggested primer 2: | TAGCTGTCTGGTGCCATCTG |