| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.248183 |
| Chromosome: | chromosome 4 |
| Location: | 1889098 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217950 | (1 of 1) PTHR24347//PTHR24349//PTHR24349:SF84 - SERINE/THREONINE-PROTEIN KINASE // SERINE/THREONINE-PROTEIN KINASE // CALCIUM-DEPENDENT PROTEIN KINASE 13 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGAGGTGTGCTGCCACTACTGGATGCAC |
| Internal bar code: | GATCCTGAGTAGTGCCGCGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 374 |
| LEAP-Seq percent confirming: | 99.4395 |
| LEAP-Seq n confirming: | 887 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGAGGCATACACTCAGCTT |
| Suggested primer 2: | TTTGCAACGGCATGAAATAA |