| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.248191 |
| Chromosome: | chromosome 10 |
| Location: | 6287614 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g465026 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCTCAATCGTGCGGACTCTGAAAGGCCG |
| Internal bar code: | AACAGCTAAGATCTTCAGGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 551 |
| LEAP-Seq percent confirming: | 99.5055 |
| LEAP-Seq n confirming: | 3823 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATGGGATGGTGTGTATCA |
| Suggested primer 2: | TTCTACCGTAACCAGCCACC |