| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.248211 |
| Chromosome: | chromosome 12 |
| Location: | 350090 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g494350 | EMP70 | Endomembrane protein 70; (1 of 1) PTHR10766:SF54 - ENDOMEMBRANE FAMILY PROTEIN 70 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGAGCCCCGATCGCAACTCCAGCCTACAG |
| Internal bar code: | GCAACGAGCGTACAGTCCGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 586 |
| LEAP-Seq percent confirming: | 99.6123 |
| LEAP-Seq n confirming: | 5395 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGCCGCTAAGCATACACG |
| Suggested primer 2: | GAGCTGTGGCTCTGGTTTTC |