| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.248235 |
| Chromosome: | chromosome 17 |
| Location: | 1535476 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g707300 | PGA3 | Phospholipid/glycerol acyltransferase; (1 of 1) PTHR23063:SF1 - 1-ACYLGLYCEROPHOSPHOCHOLINE O-ACYLTRANSFERASE 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGAACGACGTCAGGGTAGGACCCACTCT |
| Internal bar code: | ACTGACTATCACTCCGAACGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 205 |
| LEAP-Seq percent confirming: | 99.4714 |
| LEAP-Seq n confirming: | 2258 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGTACCTGATTCCGCCAC |
| Suggested primer 2: | CCTCCACTCTCTCAACTCCG |