| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.248236 |
| Chromosome: | chromosome 9 |
| Location: | 41365 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386143 | (1 of 2) 1.14.11.4 - Procollagen-lysine 5-dioxygenase / Procollagen-lysine,2-oxoglutarate 5-dioxygenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTCCGGCCAGCTTCACCACCTTGAACCG |
| Internal bar code: | AGTTTTTACTTATGCCCTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 708 |
| LEAP-Seq percent confirming: | 97.3747 |
| LEAP-Seq n confirming: | 408 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAACTCGGGTGGCTACAT |
| Suggested primer 2: | CATACACACCGCACCAAAAG |