| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.248250 |
| Chromosome: | chromosome 12 |
| Location: | 517211 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g493100 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATAGTGTGCACCGATGTCCCTACTGTTCT |
| Internal bar code: | TGGCATGCGAGGAGACTATTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 590 |
| LEAP-Seq percent confirming: | 98.9798 |
| LEAP-Seq n confirming: | 9508 |
| LEAP-Seq n nonconfirming: | 98 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGGAAGCTCGCATGTCTG |
| Suggested primer 2: | AGCGGCTCATTCTCTGACAT |