| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.248268 |
| Chromosome: | chromosome_17 |
| Location: | 3085407 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g721000 | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TACGAGAACCCGCAGAACGCAGTTTAGTTA |
| Internal bar code: | CGCGTCGCTCGGGGTCGTCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 816 |
| LEAP-Seq percent confirming: | 97.4843 |
| LEAP-Seq n confirming: | 465 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCCCAATGACTACCCCAA |
| Suggested primer 2: | GGTTCAGCAGGAGCTTGTTC |