| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.248274 |
| Chromosome: | chromosome_16 |
| Location: | 4943920 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g685450 | DHC8 | Flagellar inner arm dynein heavy chain, dynein e (monomeric) | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TAGCTAGGCAGGCAGGCAGGGCTGGCGGCA |
| Internal bar code: | GATCAAAAGACACATAATTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 654 |
| LEAP-Seq percent confirming: | 97.9556 |
| LEAP-Seq n confirming: | 1677 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTCAACACCCCAGAACAC |
| Suggested primer 2: | GACTGCATCGACGACTTCAA |