Insertion junction: LMJ.RY0402.248276_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.248276
Chromosome:chromosome 17
Location:274047
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g698150 CYCR4 Cyclin-related protein; (1 of 10) PTHR10177 - CYCLINE 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTAATTGTCGTGCGCTGTTGTGGCCTTGTG
Internal bar code:GACCAGCCCCCAGGACTAGCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:626
LEAP-Seq percent confirming:98.9314
LEAP-Seq n confirming:4166
LEAP-Seq n nonconfirming:45
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:GAGACAGGGTAAGTCGCTGC
Suggested primer 2:GACGGAGTTGGCGATAGGTA