| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.248277 |
| Chromosome: | chromosome_14 |
| Location: | 1426016 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g617700 | PDE2 | 3\',5\'-cyclic-nucleotide phosphodiesterase | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCCAATGGTATCGATGCTTGGGGCTTTTGG |
| Internal bar code: | ACCAGGGCCGCTGTTGCTCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 751 |
| LEAP-Seq percent confirming: | 99.5472 |
| LEAP-Seq n confirming: | 8134 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGGGGGTGAGTAAGGAAT |
| Suggested primer 2: | GCAGCTTCACAAAGTAGGGC |