| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.248292 |
| Chromosome: | chromosome 1 |
| Location: | 5296436 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g037050 | (1 of 3) 2.4.2.30 - NAD(+) ADP-ribosyltransferase / Poly(ADP-ribose)polymerase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCAATCTGGCGCGCCTCGTGGGCTGCGA |
| Internal bar code: | ATACTCCTTTGGTCTAAGACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1088 |
| LEAP-Seq percent confirming: | 99.4551 |
| LEAP-Seq n confirming: | 9491 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGACTATGCCAGATGCAA |
| Suggested primer 2: | CACACACACAAGTAACCCCG |