Insertion junction: LMJ.RY0402.248296_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.248296
Chromosome:chromosome 8
Location:1176437
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g363000 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCACCTTTCACTCCTCCATGCCACTCCC
Internal bar code:GAGTTCCCGATTGCACCAACAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:165
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCCCACGACTTGACACTT
Suggested primer 2:ATCGGTACACGCCAGGTAAC