Insertion junction: LMJ.RY0402.248320_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.248320
Chromosome:chromosome 2
Location:3617610
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g095141 (1 of 1) IPR002938//IPR006076 - FAD-binding domain // FAD dependent oxidoreductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGAACCCGATGCAAAAGCTGAGCTAGCGGA
Internal bar code:CGCGGATCACAAGAATCTCGAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:218
LEAP-Seq percent confirming:98.5772
LEAP-Seq n confirming:1940
LEAP-Seq n nonconfirming:28
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTGAGAGTGGGTAGGTGA
Suggested primer 2:AATACAGCTGGACACCCAGG