Insertion junction: LMJ.RY0402.248350_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.248350
Chromosome:chromosome 9
Location:1722037
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g396750 CSE18 Predicted protein of CSE family; (1 of 1) IPR000601//IPR022409 - PKD domain // PKD/Chitinase domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCACCTGCGTGGCCGGGATCGTGTGGGCC
Internal bar code:GAGATCGGACCCGTCTGCCTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:654
LEAP-Seq percent confirming:99.1286
LEAP-Seq n confirming:4323
LEAP-Seq n nonconfirming:38
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGACGCCCATGGATAACT
Suggested primer 2:GTTTGCGAGGTTGTGGAGTT