| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.248350 |
| Chromosome: | chromosome 12 |
| Location: | 9368915 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g540400 | RCD1 | Rcd1-like protein; (1 of 2) K12606 - CCR4-NOT transcription complex subunit 9 (RCD1, CNOT9, CAF40) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACATCGGGCATGGCTGGACGCCGATGCGT |
| Internal bar code: | TATAGCTCGGTGCTACTCCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 431 |
| LEAP-Seq percent confirming: | 99.1525 |
| LEAP-Seq n confirming: | 117 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTTGTGGGTCGAGCTAAT |
| Suggested primer 2: | TGTAGTACCCGACCGTGTCA |