Insertion junction: LMJ.RY0402.248352_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.248352
Chromosome:chromosome 6
Location:180202
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g250300 DHC16,DHC1b,FLA24 (1 of 1) K10414 - dynein heavy chain 2, cytosolic (DYNC2H, DNCH2); Cytoplasmic Dynein 1b Heavy Chain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTGGAGAGCACGACCTGTTCGAGGGCAC
Internal bar code:ATGATGCGTGGACATCAACAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:648
LEAP-Seq percent confirming:98.8764
LEAP-Seq n confirming:2200
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:62

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGACACCCCTTATCCTGA
Suggested primer 2:GTTCATTAAAGCTGCTCGGC