Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.248356 |
Chromosome: | chromosome 12 |
Location: | 8754256 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g545752 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGTTCCACAGCACACGCGCTGAGGCGTC |
Internal bar code: | GGCCAACCTTCATTGCGATGCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 412 |
LEAP-Seq percent confirming: | 74.9415 |
LEAP-Seq n confirming: | 640 |
LEAP-Seq n nonconfirming: | 214 |
LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGGATTGATGTGGGTCAGTC |
Suggested primer 2: | CCTGGGTTGTGATAGCACCT |