| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.248359 |
| Chromosome: | chromosome 3 |
| Location: | 2954233 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g163300 | CDT1 | (1 of 1) PTHR28637:SF1 - DNA REPLICATION FACTOR CDT1; DNA replication initiation factor, CDT1-like | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGCTTGGGGGCCTAAGTGGGACGCGGGC |
| Internal bar code: | TCGGTTGATCCTCAATCAGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 618 |
| LEAP-Seq percent confirming: | 99.5128 |
| LEAP-Seq n confirming: | 7558 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTCACCGTACAGCATCCC |
| Suggested primer 2: | TTGTCAAGCCAAAGCATCTG |