| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.248362 |
| Chromosome: | chromosome 2 |
| Location: | 1213894 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g082200 | HSK1 | (1 of 3) 2.7.1.39 - Homoserine kinase; Homoserine kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGGCAAGCAGGCAGAGGCAGGCAGGCC |
| Internal bar code: | CGGGAACCAGGATTTTAGCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 278 |
| LEAP-Seq percent confirming: | 98.9247 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGCTGGTGGGTGAGAGTC |
| Suggested primer 2: | AAACACCACGAGTGTGACCA |