| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.248367 |
| Chromosome: | chromosome 2 |
| Location: | 2883129 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g094900 | (1 of 1) PTHR23083//PTHR23083:SF427 - TETRATRICOPEPTIDE REPEAT PROTEIN, TPR // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGTAGTCGGTGACGGCGCTCTCGTACTC |
| Internal bar code: | GGGGAGCGTCGGTTAGGGTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 299 |
| LEAP-Seq percent confirming: | 95.6149 |
| LEAP-Seq n confirming: | 1003 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACCGGTTAGTGGTGTGGC |
| Suggested primer 2: | ATTGGGTCTCTCCCCAGTCT |