| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.248377 |
| Chromosome: | chromosome 14 |
| Location: | 3750944 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g632176 | (1 of 1) PTHR10641:SF660 - DNA-BINDING PROTEIN REB1-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGATCGAAAGCATAGGCGCACATGGGGG |
| Internal bar code: | TTCCTCATGCGGGATGTCTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 606 |
| LEAP-Seq percent confirming: | 99.7435 |
| LEAP-Seq n confirming: | 11666 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACATCACCAGCATCACCAA |
| Suggested primer 2: | CCTGAAGCCAGCTGTAAAGG |