| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.248379 |
| Chromosome: | chromosome 2 |
| Location: | 116134 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g074100 | (1 of 4) IPR028307 - Lin-54 family | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTTCATTTTCCGAGTACTGCTTTGGCTG |
| Internal bar code: | AAAGGTCCATTTATATTTGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 910 |
| LEAP-Seq percent confirming: | 98.917 |
| LEAP-Seq n confirming: | 2740 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGAGGTATGAGGCCAGGA |
| Suggested primer 2: | ACTGTAGCCGCTTGAATGCT |